研究成果
代表性论文
完整的论文列表请见本页末尾。
本研究提出了一种新型的超灵敏蛋白检测方法——包容性液滴数字免疫分析（iddELISA）。与传统方法只分析单颗微球的液滴不同，iddELISA结合统计模型，能够利用包含多颗微球的所有液滴，从而提高检测效率和准确性。以新冠病毒的核衣壳蛋白为例，该方法的检测灵敏度远超传统手段。该技术有望应用于极低浓度生物标志物的早期检测，对疾病早筛具有重要意义。
Yujuan Chai, Xiaoxiang Hu, Qi Fang, Yuanyuan Guo, Binmao Zhang, Hangjia Tu, and Zida Li*
Anal. Chem. 97(1), 444–453 (2025)
我们实验室开发了StratoLAMP，这是一种全新的多重基因检测方法，不依赖传统的荧光标记，因而更加简单、经济。该方法利用在DNA扩增过程中自然生成的微小沉淀物作为可视信号。通过精心设计，我们能够根据沉淀物的多少判断样本中含有哪些基因，并利用人工智能图像分析技术对结果进行高精度识别。StratoLAMP大大降低了基因检测的门槛，使其更适用于资源有限的环境，是实现更广泛分子诊断应用的重要一步。
Meichi Jin,# Jingyi Ding,# Yu Zhou, Jiazhao Chen, Yi Wang, and Zida Li*
Proc. Natl. Acad. Sci. U.S.A., 121(2), e2314030121, 2024
本文介绍了一种名为 CoID-LAMP 的新方法，可同时检测和定量多种核酸（即基因材料）。与传统复杂的荧光标记方法不同，CoID-LAMP 利用颜色编码的液滴和深度学习图像分析来识别和统计不同的基因目标。该方法通过微阵列芯片将含有不同引物的彩色液滴与样本液滴配对，使反应在芯片上进行并通过图像观察。该系统兼容荧光和非荧光（可见光）染料，降低了成本，并简化了操作流程。这种技术有望让基因检测更加快捷、简便和普及。
Kai Wu,# Qi Fang,# Zhantao Zhao, and Zida Li*
Anal. Chem. 95(11), 5069–5078 (2023)
本研究开发了一种名为CP-seq的新技术，用于在单个细胞水平上测试药物组合效果。研究人员通过给每种药物添加独特的条形码，并利用微流控装置将药物与细胞随机混合在微小液滴中。处理后，可以同时读取细胞的基因表达情况和其接触的药物组合。该方法能高效测试上千种药物组合，有望加速新疗法的发现并降低耐药性问题。
Run Xie,# Yang Liu,# Shiyu Wang, Xuyang Shi, Zhantao Zhao, Longqi Liu, Ya Liu,* and Zida Li*
Biosens. Bioelectron. 220, 114913 (2023)
本研究提出了一种名为“deep-dLAMP”的低成本核酸定量方法，结合了常规实验室设备和深度学习技术。传统数字PCR需昂贵设备生成均一液滴，而该方法通过简单的涡旋混合生成不同大小的液滴，再借助深度学习图像分析判断每个液滴中是否发生了DNA扩增反应。通过识别液滴中可见沉淀物并结合液滴体积，利用统计模型估算样本中的核酸浓度。该方法检测准确、对实验条件适应性强，有望推广应用于普通实验室或现场核酸检测中。
Linzhe Chen,# Jingyi Ding,# Hao Yuan, Chi Chen,* and Zida Li*
Adv. Sci. 9(9), 2105450 (2022)
本研究开发了一种简便的新型血液检测设备，用于监测血液凝固的速度，这在手术及疾病管理中非常重要。该设备通过将血液和缓冲液混合成微小液滴，并观察血液在凝固过程中颜色的变化来判断其粘稠程度。通过分析液滴颜色变化，就可以推断出血液的凝固功能。研究团队还设计了一款便携原型设备，显示出这种技术有望应用于诊所或紧急情况等实验室之外的场景。
Linzhe Chen, Donghao Li, Xinyu Liu, Yihan Xie, Jieying Shan, Haofan Huang, Xiaxia Yu, Yudan Chen, Weidong Zheng, and Zida Li*
ACS Sens. 7(8), 2170–2177 (2022)
专利
Zida Li, Tao Wang, Yongjin Zhou, Zengtong Chen
A system and method for single cell isolation using droplet microfluidics
China Invention Patent Provisional Application, CN113477282A (2021)
Zida Li, Jingyi Ding, Linzhe Chen, Chi Chen
A nucleic acid quantification method based on non-uniform droplets and image analysis
China Invention Patent Provisional Application, CN114540469A (2022)
Zida Li, Qi Fang, Yujun Chai, Xiaoxiang Hu
A Droplet Digital Enzyme-Linked Immunosorbent Assay Method, Device, and Related Medium
China Invention Patent Application, ZL202410149722.7 (2024)
Zida Li, Donghao Li, Yujuan Chai, Xinyu Liu, Weidong Zheng
A detection system and method for point-of-care blood clotting function assessment
China Invention Patent Application, ZL202111620552.9 (2025)
Zida Li, Linzhe Chen, Weidong Zheng, Jieying Shan, Yihan Xie, Xinyu Liu
A device and method for the assessement of blood viscosity using microfluidics
China Invention Patent Application, ZL202110639057.6 (2021)
Zida Li, Meichi Jin, Jingyi Ding, Yi Wang, Yu Zhou
The Multiplex Nucleic Acid Quantification Method, Device, and Medium Based on Precipitation Bright Field Image Processing
China Invention Patent Application, ZL202310028332.X (2022)
Zida Li, Zhantao Zhao
An image-activated picoinjection method, system, and equipment
China Invention Patent Application, ZL202211449260.8 (2023)
Zida Li, Qi Fang, Kai Wu
Method, device, and medium for multiple digital detection of nucleic acid with deep learning
China Invention Patent Application, ZL202211516857.X (2023)
Zida Li, Xiaxia Yu, Xinyu Liu, Jieying Shan, Yihan Xie
Simulation system and method of in vitro diagnostics
China Invention Patent Application, ZL202110750662.0 (2023)
Zida Li, Lanzhu Huang, Weidong Zheng
A fabrication method and application of soft post rings for clot retraction testing
China Invention Patent Application, ZL202010260648.8 (2022)
Jianping Fu, Kevin Ward, Zida Li, Xiang Li
A microscale device for blood coagulation assay
U.S. Patent Application, 62/304,385 (2018)
Ho Cheung Shum, Alban Sauret, Zida Li, Yang Song
System and method for generation of emulsions with low interfacial tension and measuring frequency vibrations in the system
U.S. Patent Application, 13/839,072 (2013)
完整论文列表
Flow-LAMP: Label-free Digital LAMP using Scatter-based Flow Cytometry on Vortex-Generated Polydisperse Gel Beads
Wanjun Yao, Yuchong Zheng, Zerui Wu, Liqun He, Weidong Zheng, and Zida Li
Submitted
Precipitate-informed deep learning enables label-free multiplex digital nucleic acid testing in polydisperse droplets
Jiazhao Chen, Jingyi Ding, Rui Deng, Yi Wang, and Zida Li
Submitted
From Droplets to Diagnosis: AI-Driven Imaging and System Integration in Digital Nucleic Acid Amplification Testing
Yuanyuan Wei, Xianxian Liu, Yao Mu, Changran Xu, Guoxun Zhang, Tianhao Li, Zida Li, Wu Yuan, Ho-Pui Ho, and Mingkun Xu
Biosensors and Bioelectronics, in press (2025)
Materials-Driven Innovations in Digital Nucleic Acid Amplification Technologies: Transforming Molecular Diagnostics
Jiazhao Chen, Kai Wu, Jingyi Ding, and Zida Li
Chemical Engineering Journal, 519, 165127, (2025)
Reimagining POCT assays: Automated digital microfluidics for multiplex in vitro diagnostics
Wenkai Fan, Donghao Li, Jingyi Ding, Zida Li*
Talanta, 294, 128270 (2025)
Droplet digital PCR-based single aptamer selection
Zerui Wu,# Wanjun Yao,# Jinyu Chen, Yonghao Chen, Zida Li, Weiping Ding,* Liqun He,* and Peng Hu*
Talanta, 127924 (2025)
Embracing Poisson encapsulation statistics for improved droplet digital immunoassay
Yujuan Chai, Xiaoxiang Hu, Qi Fang, Yuanyuan Guo, Binmao Zhang, Hangjia Tu, and Zida Li*
Anal. Chem. 97(1), 444–453 (2025)
StratoLAMP: Label-free, multiplex digital loop-mediated isothermal amplification based on visual stratification of precipitate
Meichi Jin,# Jingyi Ding,# Yu Zhou, Jiazhao Chen, Yi Wang, and Zida Li*
Proc. Natl. Acad. Sci. U.S.A., 121(2), e2314030121, 2024
Image-activated pico-injection for single cell analysis
Zhantao Zhao, Heng Zhai, Peng Zuo, Tao Wang, Run Xie, Mu Tian, Ruyuan Song, Xiaonan Xu, and Zida Li*
Talanta 272, 125765 (2024)
CoID-LAMP: Color-encoded, intelligent digital LAMP for multiplexed nucleic acid quantification
Kai Wu,# Qi Fang,# Zhantao Zhao, and Zida Li*
Anal. Chem. 95(11), 5069–5078 (2023)
Combinatorial perturbation sequencing on single cells using microwell-based droplet random pairing
Run Xie,# Yang Liu,# Shiyu Wang, Xuyang Shi, Zhantao Zhao, Longqi Liu, Ya Liu,* and Zida Li*
Biosens. Bioelectron. 220, 114913 (2023)
High resolution, multiplex antibody patterning using micropillar-focused droplet printing and microcontact printing
Meichi Jin, Kai Wu, Mengzhen Wang, Yang Zhang, Chengbin Yang, and Zida Li*
Adv. Biol. 7(8), 2300111 (2023)
Bone-on-a-chip platforms and integrated biosensors: towards advanced in vitro bone models with real-time biosensing
Yang Zhang, Taozhao Yu, Jingyi Ding, and Zida Li*
Biosens. Bioelectron. 219, 114798 (2023)
deep-dLAMP: deep learning-enabled polydisperse emulsion-based digital loop-mediated isothermal amplification
Linzhe Chen,# Jingyi Ding,# Hao Yuan, Chi Chen,* and Zida Li*
Adv. Sci. 9(9), 2105450 (2022)
Point-of-care blood coagulation assay based on dynamic monitoring of blood viscosity using droplet microfluidics
Linzhe Chen, Donghao Li, Xinyu Liu, Yihan Xie, Jieying Shan, Haofan Huang, Xiaxia Yu, Yudan Chen, Weidong Zheng, and Zida Li*
ACS Sens. 7(8), 2170–2177 (2022)
Generation and Screening of Antigen-Specific Nanobodies from Mammalian Cells Expressing the BCR Repertoire Library Using Droplet-Based Microfluidics
Menghua Lyu,# Xuyang Shi,# Xiaopan Liu,# Yang Liu, Xijun Zhu, Lijuan Liao, Hongyan Zhao, Na Sun, Shiyu Wang, Linzhe Chen, Linyuan Fan, Qumiao Xu, Qianqian Zhu, Kai Gao, Huaying Chen, Yonggang Zhu, Zida Li, Weijin Guo, Yue Zheng, Ying Gu, Longqi Liu,* Meiniang Wang,* and Ya Liu*
Anal. Chem. 94(22), 7970–7980 (2022)
Point-of-care blood coagulation assay enabled by printed circuit board-based digital microfluidics
Donghao Li,# Xinyu Liu,# Yujuan Chai,# Jieying Shan, Yihan Xie, Yong Liang, Susu Huang, Weidong Zheng, and Zida Li*
Lab Chip 22(4), 709-716 (2022)
Single-cell sequencing to unveil the mystery of embryonic development
Zida Li,#,* Feng Lin,# Chu-Han Zhong, Shue Wang, Xufeng Xue, and Yue Shao*
Adv. Biol. 6(2), 2101151 (2022)
High-throughput functional screening of antigen-specific T-cells based on droplet microfluidics on single-cell level
Shiyu Wang,# Yang Liu,# Yijian Li, Menghua Lv, Kai Gao, Ying He, Wenbo Wei, Yonggang Zhu, Xuan Dong, Xun Xu, Zida Li,* Longqi Liu,* and Ya Liu*
Anal. Chem. 94(2), 918–926 (2022)
Emerging biosensing technologies for improved diagnostics of COVID-19 and future pandemics
Linzhe Chen, Guoliang Zhang, Longqi Liu, and Zida Li
Talanta 225, 121986 (2021)
Micro-engineered flexural post rings for effective blood sample fencing and high throughput measurement of clot retraction force
Lanzhu Huang,# Xinyu Liu,# Yuanbin Ou, Haofan Huang, Xia Zhang, Yize Wang, Yong Liang, Xiaxia Yu, Weidong Zheng, Huisheng Zhang, and Zida Li*
ACS Sens. 5(12), 3949-3955 (2020)
Recent advances in solar-driven evaporation system
Zhourui Xu, Zida Li, Yihang Jiang, Gaixia Xu, Mingwei Zhu, Wing-Cheung Law, Ken-Tye Yong, Yanshuai Wang, Chengbin Yang, Biqin Dong, and Feng Xing*
J. Mater. Chem. A 8, 25571-25600 (2020)
Stretchable supercapacitors as emergent energy storage units for health monitoring bioelectronics
Xue Chen, Nicolo Simone Villa, Yanfeng Zhuang, Linzhe Chen, Tianfu Wang, Zida Li, and Tiantian Kong
Adv. Energy Mater. 10(4), 1902769 (2020)
Dean flow assisted single cell and bead encapsulation for high performance single cell expression profiling
Luoquan Li,# Ping Wu,# Zhaofeng Luo, Lei Wang, Weiping Ding, Tao Wu, Jinyu Chen, Jinlong He, Yi He, Heran Wang, Ying Chen, Guibo Li, Zida Li,* and Liqun He*
ACS Sens. 4(5), 1299-1305 (2019)
Multiple splitting of droplets using multi-furcating microfluidic channels
Zida Li, Luoquan Li, Meixiang Liao, Liqun He, and Ping Wu
Biomicrofluidics 13(2), 024112 (2019)
Controlled modeling of human epiblast and amnion development using stem cells
Yi Zheng, Xufeng Xue, Yue Shao, Sicong Wang, Sajedeh Nasr Esfahani, Zida Li, Jonathon M. Muncie, Johnathon N. Lakins, Valerie M. Weaver, Deborah L. Gumucio, and Jianping Fu*
Nature 573(7774), 421-425 (2019)
Dorsal-ventral patterned neural cyst from human pluripotent stem cells in a neurogenic niche
Yuanyuan Zheng,# Xufeng Xue,# Agnes M. Resto Irizarry, Zida Li, Yue Shao, Yi Zheng, Gang Zhao,* and Jianping Fu*
Sci. Adv. 5(12), eaax5993 (2019)
Microengineered human amniotic ectoderm tissue array for high-content developmental phenotyping
Sajedeh Nasr Esfahani, Yue Shao, Agnes M Resto Irizarry, Zida Li, Xufeng Xue, Deborah L Gumucio, and Jianping Fu*
Biomaterials 216, 119244 (2019)
Biophysical phenotypes and determinants of anterior vs. posterior primitive streak cells derived from human pluripotent stem cells
Feng Lin, Yue Shao, Xufeng Xue, Yi Zheng, Zida Li, Chunyang Xiong, and Jianping Fu
Acta Biomater. 86, 125-134 (2019)
Carbon nanotube strain sensor based hemoretractometer for blood coagulation testing
Zida Li, Yize Wang, Xufeng Xue, Brendan McCracken, Kevin Ward, and Jianping Fu*
ACS Sens. 3(3), 670-676 (2018)
Capillary assisted deposition of carbon nanotube film for strain sensing
Zida Li, Xufeng Xue, Feng Lin, Yize Wang, Kevin Ward, and Jianping Fu*
Appl. Phys. Lett. 111(17), 173105 (2017)
Tracking the tumor invasion front using long-term fluidic tumoroid culture
Koh Meng Aw Yong, Zida Li, Sofia D. Merajver, and Jianping Fu*
Sci. Rep. 7, (2017)
Acoustic tweezing cytometry enhances osteogenesis of human mesenchymal stem cells through cytoskeletal contractility and YAP activation
Xuefeng Xue, Xiaowei Hong, Zida Li, Cheri X. Deng, and Jianping Fu*
Biomaterials 134, 22-30 (2017)
Controlled tubular unit formation from collagen film for modular tissue engineering
Jianming Sang, Xiang Li, Yue Shao, Zida Li, and Jianping Fu*
ACS Biomater. Sci. Eng. 3(11), 2860-2868 (2016)
A miniaturized hemoretractometer for blood clot retraction testing
Zida Li, Xiang Li, Brendan McCracken, Yue Shao, Kevin Ward, and Jianping Fu*
Small 12, 3926–3934 (2016)
Musical interfaces: visualization and reconstruction of music with a microfluidic two-phase flow
Sze Yi Mak, Zida Li, Arnaud Frere, Tat Chuen Chan, and Ho Cheung Shum*
Sci. Rep. 4, 6675 (2014)
Syringe-pump-induced fluctuation in all-aqueous microfluidic system implications for flow rate accuracy
Zida Li, Sze Yi Mak, Alban Sauret, and Ho Cheung Shum*
Lab Chip 14(4), 744-749 (2014)
Emerging microengineered tools for functional analysis and phenotyping of blood cells
Xiang Li, Weiqiang Chen, Zida Li, Ling Li, Hongchen Gu, and Jianping Fu*
Trends Biotechnol. 32(11), 586-594 (2014)
Dynamics for dense packing of colloids
Liqun He*, Ping Wu, Zida Li, and Lili Feng
Adv. Mater. Res. 165, 248-254 (2012)